HPV is implicated in laryngeal cancer

Human papillomavirus (HPV) infection interferes with DNA repair and the destruction of cells with unrepairable defective DNA. This means that mutated cells survive to become cancers, instead of being eliminated. The virus also directly disrupts the cell nucleus. HPV has been found in a proportion of laryngeal cancers, an alleged smoking-related cancer. As was the case with cervical cancer, it is likely that as methodology improves, the proportion of laryngeal cancer in which HPV is implicated will increase with time. The majority of this work has only been performed since 1996, and the US health establishment has had relatively little involvement.

The tobacco industry funded some of the pioneering investigations of Syrjanen and Syrjanen. The absence of interest and support from the health establishment accounts for their seeking support from this source. History now shows that the Syrjanens were right to suspect a role for HPV. In 1989, a research analyst for Shook, Hardy & Bacon noted that the industry position paper on cancer of the larynx ignored HPV ("The one theory of causation which is noticeably absent from Mr. Goold's paper is that of human papillomavirus (HPV) as an etiologic agent for some squamous cell carcinomas of the larynx..."). But, despite ironclad proof that the anti-smokers falsely blamed smoking for cervical cancer that was actually caused by HPV, the tobacco lawyers have never uttered so much as a word about this subject. This is conspiracy. at the Minnesota tobacco trial, the tobacco industry said nothing about HPV. For obvious reasons, neither did Jonathan Samet, the anti-smokers' star perjuror about smoking and health. Furthermore, these facts cast a whole different light on those demonstrations against the tobacco companies that the anti-smoking demagogues arranged: The anti-smokers are to blame for their misfortune, because they are the ones who obstructed the truth!

Histological evidence for the presence of condylomatous epithelial lesions in association with laryngeal squamous cell carcinoma. KJ Syrjanen, SM Syrjanen. ORL J Otorhinolaryngol Relat Spec 1981;43(4):181-194. "The role of HPV in the development of these lesions is discussed, and the possibility is raised that HPV might be the agent responsible for contributing to the development of squamous cell carcinoma of the larynx in man."

Syrjanen - ORL Otorhinolaryngol Relat Spec 1981 abstract / PubMed

Human papilloma virus (HPV) antigens in lesions of laryngeal squamous cell carcinomas. K Syrjänen, S Syrjänen, S Pyrhönen. ORL J Otorhinolaryngol Relat Spec 1982;44(6):323-334. 36% of 36 laryngeal squamous cell carcinomas with histological features of coexistent condylomatous changes showed HPV antigens.

Syrjänen - ORL J Otorhinolaryngol Relat Spec 1982 abstract / PubMed

Analysis of prevalence of human papillomavirus in laryngeal carcinomas. Study of 40 cases using polymerase chain reaction and consensus primers. MS Brandwein, GJ Nuovo, H Biller. Ann Otol Rhinol Laryngol 1993 Apr;102(4 Pt 1):309-313. 3 of 40 patients (8%) had detectable HPV DNA, by polymerase chain reaction (PCR) with the "hot start" modification and consensus primers that can detect over 30 distinct HPV types.

Brandwein - Ann Otol Rhinol Laryngol 1993 abstract / PubMed

High frequency of p53 abnormality in laryngeal cancers of heavy smokers and its relation to human papillomavirus infection. T Suzuki, K Shidara, F Hara, T Nakajima. Jpn J Cancer Res 1994 Nov;85(11):1087-1093. 11/41 (27%) laryngeal cancers were positive for HPV 16/18.

Suzuki - Jpn J Cancer Res 1994 abstract / PubMed

Prevalence of human papillomaviruses (HPV) in benign and malignant tumors of the upper respiratory tract. J Shen, JE Tate, CP Crum, ML Goodman. Mod Pathol 1996 Jan;9(1):15-20. 3/32 (9%) laryngeal squamous cell carcinomas were positive for HPV types 6 and 11.

Shen - Mod Pathol 1996 abstract / PubMed

Detection of human papillomavirus DNA in laryngeal squamous cell carcinoma by polymerase chain reaction. G Almadori, G Cadoni, P Cattani, P Posteraro, E Scarano, F Ottaviani, G Paludetti, M Maurizi. Eur J Cancer 1996 May;32A(5):783-788. Nine (20%) of 45 patients were positive, by PCR with consensus primers that detect HPV types 6, 11, 16 and 18.

Almadori - Eur J Cancer 1996 abstract / PubMed

Human papillomavirus and cancers of the upper aerodigestive tract: a review of epidemiological and experimental evidence. S Franceschi, N Munoz, XF Bosch, PJ Snijders, JM Walboomers. Cancer Epidemiol Biomarkers Prev 1996 Jul;5(7):567-575. "Increased risk of cancer of the oral cavity, pharynx, and larynx subsequent to the occurrence of cancer of the cervix has been found and suggests common etiological factors besides smoking. HPV has been found in a substantial proportion of benign UADT lesions, most notably laryngeal papillomas and oral verrucal-papillary lesions. Largest and most accurate case series (i.e., > 15 UADT cancer cases, based on best HPV detection techniques) showed HPV DNA in 46% of cancers of the oral cavity and pharynx, 15% of cancers of the esophagus, and 24% of cancers of the larynx, with however, great discrepancies from one study to another."

Franceschi - Cancer Epidemiol Biomarkers Prevent 1996 abstract / PubMed
Franceschi / Cancer Epidemiol Biomarkers Prevent 1996 full article (pdf, 9 pp)

Presence of human papillomavirus in squamous cell laryngeal carcinomas. A study of thirty-nine cases using polymerase chain reaction and in situ hybridization. ES Lie, F Karison, R Holm. Acta Otolaryngol 1996 Nov;116(6):900-905. 3/39 (8%) were positive for HPV, with specific PCR primers for HPV 6, 11, 16, 18, 31, 33 and 35 and with in situ hybridization (ISH). One was HPV 16 and the other two were "type related to HPV 6/11."

Lie - Acta Otolaryngol 1996 abstract / PubMed

[The detection of human papillomavirus in papillomas of the larynx and tonsils through immunohistochemistry and DNA in situ hybridization]. M Lopez Amado, T Garcia Caballero, A Lozano Ramirez, T Labella Caballero. An Otorrinolaringol Ibero Am 1997;24(3):269-280. 5/10 laryngeal papillomas were HPV-positive; two of these patients later developed a laryngeal carcinoma.

Lopez Amado - An Otorrinolaringol Ibero Am 1997 abstract / PubMed

[A preliminary study on p53 gene expression and infection of human papilloma virus in laryngeal squamous cell carcinoma]. M Xie, J Xiao, Z Tao, J Luo. Hunan I Ko Ta Hsueh Hsueh Pao 1997;22(3):209-211. 3/13 (23.1%) laryngeal squamous cell carcinomas were positive for HPV type 16; no HPV 18 reported.

Xie - Hunan I Ko Ta Hsueh Hsue Pao 1997 abstract / PubMed

[Study on the relation between HPV and tumors of the throat and larynx]. J Ren, X Wang, Q Zhu. Lin Chuong Erh Pi Yen Hou Ko Tsa Chih 1997 Apr;11(4):157-159. 6/22 (27.3%) of laryngeal squamous cell carcinomas were positive for HPV DNA (HPV types 6, 11, 16, or 18).

Ren - Lin Chuang Erh Pi Yen Hou Ko Tsa Chih 1997 abstract / PubMed

[Detection of human papillomavirus gene sequences in laryngeal tumors and premalignant changes by polymerase chain reaction]. J Czegledy, T Major, A Juhasz, G Repassy, L Gergely. Orv Hetil 1997 Jul 27;138(30):1891-1895. "In the squamous cell carcinomas, papillomas and precancerous lesions the presence of human papillomavirus gene sequences was significantly higher than in the control group." (HPV types 6, 11, 16 and 18).

Czegledy - Orv Hetil 1997 abstract / PubMed

Using polymerase chain reaction to human papillomavirus in oral and pharyngolaryngeal carcinomas. I Alvarez Alvarez, P Sanchez Lazo, S Ramos Gonzalez, JP Rodrigo Tapia, F Nunez Batalla, C Suarez Nieto. Am J Otolaryngol 1997 Nov-Dec;18(6):375-381. "HPV E6 DNA of the 6b and 16 types was detected in 14 patients (25%)," but not HPV 18.

Alvarez - Am J Otolaryngol 1997 abstract / PubMed

Localization of p53 protein and human papillomavirus in laryngeal squamous lesions. ML Caruso, AM Valentini. Anticancer Res 1997 Nov-Dec;17(6D):4671-4675. 1/12 (8%) laryngeal squamous cell carcinomas was positive for HPV16 or 18.

Caruso - Anticancer Res 1997 abstract / PubMed

[Study on HPV infection and p53 protein expression in laryngeal carcinoma]. Z Qin, M Dong. Lin Chuang Erh Pi Yen Hou Ko Tsa Chih 1997 Dec;11(12):546-549. 19/44 (43.2%) of laryngeal squamous cell carcinomas were positive for HPV 16/18.

Qin - Lin Chuang Erh Pi Yen Hou Ko Tsa Chih 1997 abstract / PubMed

Low detection rate of HPV in oral and laryngeal carcinomas. T Matzow, M Boysen, M Kalantari, B Johansson, B Hagmar. Acta Oncol 1998;37(1):73-76. 3/16 (19%) laryngeal carcinomas and dysplasias were positive for HPV.

Matzow - Acta Oncol 1998 abstract / PubMed

Human papillomavirus DNA sequences and p53 over-expression in laryngeal squamous cell carcinomas in Northeast China. XL Ma, K Ueno, ZM Pan, sz Hi, M Ohyama, Y Eizuru. J Med Virol 1998 Mar;54(3):186-191. 60/102 (58.8%) were positive for HPV types 16, 18, 6, 11 or 33.

Ma - J Med Virol 1998 abstract / PubMed

Malignant transformation of recurrent respiratory papillomatosis associated with integrated human papillomavirus type 11 DNA and mutation of p53. PL Rady, VJ Schnadig, RL Weiss, TK Hughes, SK Tyring. Laryngoscope 1998 May;108(5):735-740. "[T]he p53 genetic mutation was associated with integration of HPV-11 in histologically malignant lesions."

Rady - Laryngoscope 1998 abstract / PubMed

Detection and typing of human papillomavirus DNA in benign and malignant tumours of laryngeal epithelium. R García-Milián, H Hernández, L Panadé, C Rodríguez, N González, C Valenzuela, MD Araña, SE Perea. Acta Otolaryngol 1998 Sep;118(5):754-758. "Four normal laryngeal samples (16%) were positive for HPV DNA against the 24 samples (82%) (p < 0.001) found for laryngeal papilloma and 16 (48.5%) (p < 0.05) found for laryngeal squamous cell carcinoma. HPV 16 was the type most frequently found in laryngeal carcinoma samples. Our results support an etiologic role for this type of HPV in the pathogenesis of laryngeal carcinoma."

García-Milián - Acta Otolaryngol 1998 abstract / PubMed

Association between cyclin D1 (CCND1) gene amplification and human papillomavirus infection in human laryngeal squamous cell carcinoma. P Cattani, S Hohaus, A Bellacosa, M Genuardi, S Cavallo, V Rovella, G Almadori, G Cadoni, J Galli, M Maurizi, G Fadda, G Neri. Clin Cancer Res 1998 Nov;4(11):2585-2589. "HPV DNA was detected in 22 of 75 (29.3%) tumors, and it belonged almost exclusively to the highly oncogenic HPV-16, HPV-18, and HPV-33."

Cattani - Clin Cancer Res 1998 abstract / PubMed
Cattani / Clin Cancer Res 1998 full article (pdf, 5 pp)

Human papilloma virus (HPV) type 16 and 18 detected in head and neck squamous cell carcinoma. H Mineta, T Ogino, HM Amano, Y Ohkawa, K Araki, S Takebayashi, K Miura. Anticancer Res 1998 Nov-Dec;18(6B):4765-4768. 10/26 (38%) laryngeal squamous cell carcinomas were positive for HPV16/18.

Mineta - Anticancer Res 1998 abstract / PubMed

Human papilloma virus (HPV) is possibly involved in laryngeal but not in lung carcinogenesis. VG Gorgoulis, P Zacharatos, A Kotsinas, A Kyroudi, AN Rassidakis, JA Ikonomopoulos, C Barbatis, CS Herrington, C Kittas. Hum Pathol 1999 Mar;30(3):274-283. HPV DNA was found in 19/91 (21%) laryngeal squamous cell carcinomas.

Gorgoulis - Hum Pathol 1999 abstract / PubMed

Detection of human papillomavirus (HPV) in laryngeal carcinoma lines provides evidence for a heterogeneic cell population. S Atula, R Grenman, H Kujari, S Syrjanen. Eur J Cancer 1999 May;35(5):825-832. 7/27 (26%) cell lines and 7/12 (58%) tumor samples were positive for high-risk types of HPV.

Atula - Eur J Cancer 1999 abstract / PubMed

Demonstration of multiple HPV types in laryngeal premalignant lesions using polymerase chain reaction and immunohistochemistry. B Azzimonti, L Hertel, P Aluffi, F Pia, G Monga, M Zocchi, S Landolfo, M Gariglio. J Med Virol 1999 Sep;59(1):110-116. "The presence of HPV DNA was detected in 28 of 50 specimens (56%), including 9/12 cases with mild dysplasia (75%), 3/6 cases with moderate dysplasia (50%), and 7/11 cases with severe dysplasia (64%). Multiple HPV infections, containing two or three types, were detected in 17 of the 28 HPV-positive lesions (60%). Of 21 cases with keratosis and no dysplasia, 11 were positive for HPV DNA (52%) and 4 showed L1 staining (36%). By contrast, L1 positivity was revealed only in two lesions with moderate dysplasia, confirming that fully productive HPV infection is strictly dependent on epithelial differentiation and surface keratinization. The probability that HPV is a cofactor in the malignant progression of these lesions is suggested by the fact that 3/4 patients who developed cancer within 50 months were positive for HPV DNA."

Azzimonti - J Med Virol 1999 abstract / PubMed

Laryngeal cancer and human papillomavirus: HPV is absent in the majority of laryngeal carcinomas. H Lindeberg, A Krogdahl. Cancer Lett 1999 Nov 1;146(1):9-13. 1/30 laryngeal carcinomas from patients without pre-existing recurrent laryngeal papillomatosis were HPV+. "The HPV type present could not be determined, but it was not type 6, 11, 13, 16, 18, 30, 31, 33, 35 or 45."

Lindeberg - Cancer Lett 1999 abstract / PubMed

Human papillomavirus in head and neck carcinomas: prevalence, physical status and relationship with clinical/pathological parameters. G Badaracco, A Venuti, R Morello, A Muller, ML Marcante. Anticancer Res 2000 Mar-Apr;20(2B):1301-1305. Of 66 tumors from various sites including 22 laryngeal squamous cell carcinomas, 24 were HPV-positive. "HPV 16 was integrated in 7/12 positive tumours without site-specificity. HPV infection was not related to age, gender, tumour stage, differentiation grade, and use of alcohol and/or tobacco."

Badaracco - Anticancer Res 2000 abstract / PubMed

Physical state and expression of human papillomavirus in laryngeal carcinoma and surrounding normal mucosa. A Venuti, V Manni, R Morello, F De Marco, F Marzetti, ML Marcante. J Med Virol 2000 Apr;60(4):396-402. 13/25 (52%) were HPV-positive: 7 for HPV-16, 5 for HPV-6, and 1 for HPV-45.

Venuti - J Med Virol 2000 abstract / PubMed

[Human papillomavirus (HPV) in the laryngeal carcinoma]. W Makowska, M Malejczyk, D Kapiszewska, J Nyckowska, E Wojcikiewicz, B Wroblewska. Otolaryngol Pol 2001;55(3):263-266. 10/23 (43.5%) were positive for HPV.

Makowska - Otolaryngol Pol 2001 abstract / PubMed

Prevalence of human papillomavirus (HPV) DNA in larynx and lung carcinomas. H Kaya, E Kotlioglu, S Inanli, G Ekicioglu, SU Bozkurt, A Tutkun, S Kullu. Pathologica 2001 Oct;93(5):531-534. 10/21 (47.6%) laryngeal SCCs were positive for HPV. "Typing showed signals of HPV 6/11, 16/18 and 31/33 infection in 80%, 40%, 30% of the laryngeal carcinomas, respectively."

Kaya - Pathologica 2001 abstract / PubMed

Prevalence, distribution, and viral load of human papillomavirus 16 DNA in tonsillar carcinomas. JP Klussmann, SJ Weissenborn, U Wieland, V Dries, J Kolligs, M Jungehuelsing, HE Eckel, HP Dienes, HJ Pfister, PG Fuchs. Cancer 2001 Dec 1;92(11):2875-2884. "Altogether 25 HNSCCs (26%) were found to be HPV positive. Stratified according to the tumor localization, the frequency of HPV positive lesions was 18% in the oral cavity, 45% for oropharynx, 25% for hypopharynx, 8% for nasopharynx, and 7% for larynx. The highest HPV DNA prevalence (58%) was found in tonsillar carcinomas."

Klussmann - Cancer 2001 abstract / PubMed

Human papillomavirus infection and epidermal growth factor receptor expression in primary laryngeal squamous cell carcinoma. G Almadori, G Cadoni, P Cattani, J Galli, F Bussu, G Ferrandina, G Scambia, G Fadda, M Maurizi. Clin Cancer Res 2001 Dec;7(12):3988-3993. HPV DNA was found in 15/42 (35.7%), nearly all of high-risk types HPV-16, HPV-18, and HPV-33. "At analysis by Mann-Whitney nonparametric statistical test, EGFR level was found to be significantly higher in HPV-infected than in HPV-negative cases (T = 440; P = 0.002). EGFR overexpression (EGFR-positive status >6 fmol/mg protein, the arbitrary cutoff value chosen) was found in 20 of 42 (47.6%) tumors, and it was associated with HPV infection in a statistically significant extent (2 = 4.686; P = 0.03). Conclusions: Viral oncoproteins have been shown to induce a perturbation of the cell response to signals for growth and differentiation; these findings confirm that enhanced EGFR expression and activation in laryngeal squamous cell carcinoma may occur also as a consequence of HPV infection and support the hypothesis of an involvement of HPV infection in laryngeal carcinogenesis."

Almadori - Clin Cancer Res 2001 abstract / PubMed
Almadori - Clin Cancer Res 2001 Full Article

[Causal association between human papilloma virus infection and head and neck and esophageal squamous cell carcinoma]. Z Szentirmay, I Szántó, I Bálint, K Pólus, E Remenár, L Tamás, G Szentkúti, Z Melegh, P Nagy, M Kásler. Magy Onkol 2002;46(1):35-41. "Overall, HPV sequences were detected in 61 of 150 specimens. HPV DNA sequences were detected in 16/32 specimens in the oropharyngeal region, in 13/36 specimens in larynx and 32/82 specimens in esophagus. Papillomas contained only the episomal form of HPV 16. In the esophagus, the most common type was HPV 73. In all specimens examined, HPV 6/11 (4/150), HPV 16 (23/150), HPV 35 (1/150), HPV 45 (1/150), HPV 54 (1/150), HPV 58 (1/150), HPV 61 (1/150), HPV 66 (1/150), HPV 68 (2/150), HPV 70 (3/150), HPV 72 (1/150), HPV 73 (16/150), double HPV infection (2/150), and unidentified HPV type (4/150) was detected. Interestingly, HPV was found in all verrucous carcinomas and in 18/22 basaloid squamous cell carcinomas."

Szentirmay - Magy Onkol 2002 abstract / PubMed

Cellular manifestations of human papillomavirus infection in laryngeal tissues. SE Jacob, S Sreevidya, E Chacko, MR Pillai. J Surg Oncol 2002 Mar;79(3):142-150. 15/44 (34%) invasive cancers were HPV positive.

Jacob - J Surg Oncol 2002 abstract / PubMed

Human papillomavirus-positive tonsillar carcinomas: a different tumor entity? JP Klussmann, SJ Weissenborn, U Wieland, V Dries, HE Eckel, HJ Pfister, PG Fuchs. Med Microbiol Immunol (Berl) 2003 Aug;192(3):129-132. 25% of hypopharyngeal cancers and 7% of laryngeal cancers were HPV DNA positive.

Klussmann - Med Microbiol Immunol (Berl) 2003 abstract / PubMed

Prevalence of human papillomavirus type 16 DNA in squamous cell carcinoma of the palatine tonsil, and not the oral cavity, in young patients: a distinct clinicopathologic and molecular disease entity. SK El-Mofty, DW Lu. Am J Surg Pathol 2003 Nov;27(11):1463-1470. In 33 patients under the age of 40 years, HPV DNA was detected by polymerase chain reaction in 0/15 oral, 10/11 tonsillar, and 2/7 laryngeal tumors. 11/12 HPV-positive tumors were HPV16 and 1 was HPV31.

El-Mofty - Am J Surg Pathol 2003 abstract / PubMed

A systematic review of case-control studies of human papillomavirus infection in laryngeal squamous cell carcinoma. L Rees, M Birchall, M Bailey, S Thomas. Clin Otolaryngol Allied Sci 2004 Aug;29(4):301-306. Review. "The studies are heterogeneous in the methods used to harvest tissue samples and techniques for detecting the virus within the tissue. HPV-16 positivity among cases ranged from 2.7% to 46.9% and 0-5.7% among controls. Two studies showed a significantly increased risk of LSCC if HPV-16 was present (OR 18.5, 95% CI 2.2-154.8, OR 2.6, 95% CI 1.1-6.0)."

Rees - Clin Otolaryngol Allied Sci 2004 abstract / PubMed

Integration of human papillomavirus type 11 in recurrent respiratory papilloma-associated cancer. PM Reidy, HH Dedo, R Rabah, JB Field, RH Mathog, L Gregoire, WD Lancaster. Laryngoscope 2004 Nov;114(11):1906-1909. "HPV-11 but not HPV-6, 16, or 18 was found in all of the laryngeal and bronchogenic cancers in patients with a history of early onset RRP in this study... HPV type 6 and 11 are considered "low-risk" viruses and are not associated with genital cancers, as are HPV types 16 and 18. However, our data suggests that HPV type 11 is an aggressive virus in laryngeal papilloma that should be monitored in patients with RRP."

Reidy - Laryngoscope 2004 abstract / PubMed

Altered patterns of the interferon-inducible gene IFI16 expression in head and neck squamous cell carcinoma: immunohistochemical study including correlation with retinoblastoma protein, human papillomavirus infection and proliferation index. B Azzimonti, M Pagano, M Mondini, M De Andrea, G Valente, G Monga, M Tommasino, P Aluffi, S Landolfo, M Gariglio. Histopathology 2004 Dec;45(6):560-572. "HPV DNA was found in 14 of 25 (56%) laryngeal SCCs and in five of nine (56%) tonsillar SCC specimens examined."

Azzimonti - Histopathology 2004 abstract / PubMed

High co-prevalence of genogroup 1 TT virus and human papillomavirus is associated with poor clinical outcome of laryngeal carcinoma. G Szladek, A Juhasz, G Kardos, K Szoke, T Major, I Sziklai, I Tar, I Marton, J Konya, L Gergely, K Szarka. J Clin Pathol 2005 Apr;58(4):402-405. 40 healthy individuals, 10 patients with recurrent papillomatosis, five patients with papillomatosis with malignant transformation, and 25 patients with laryngeal carcinoma. "In the 11 patients with carcinoma who had metastasis or relapse there was a high rate of coinfection with genogroup 1 TTV and HPV (eight of 11), whereas in the 14 without tumour progression no coinfection was found. Coinfection was associated with significantly lower tumour free survival in patients with carcinoma (p < 0.001). Furthermore, four of five patients who had papillomatosis with malignant transformation were coinfected with genogroup 1 TTV and HPV."

Szladek - J Clin Pathol 2005 abstract / PubMed
Szladek / J Clin Pathol 2005 full article

Molecular detection and typing of human papillomavirus in laryngeal carcinoma specimens. MC Torrente, S Ampuero, M Abud, JM Ojeda. Acta Otolaryngol 2005 Aug;125(8):888-893. "Ten of the 31 samples (32%) were positive for HPV DNA and all of the samples were positive for human beta-globin. The genotypes identified were HPV 16 (n=3), HPV 58 (n=2) and HPV 39, 45, 51, 59, 66 and 69 (n=1 for each). The three samples positive for HPV 16 had lost region E2, meaning that the viral DNA had been integrated into the host genome."

Torrente - Acta Otolaryngol 2005 abstract / PubMed

[The prevalence of E6/E7 HPV type 16 in laryngeal cancer and in normal mucosa] K Morshed, M Polz-Dacewicz, B Rajtar, M Szymanski, M Ziaja-Soltys, W Golabek. Pol Merkur Lekarski 2005 Sep;19(111):291-293. HPV 16 DNA was found in 26 (36.1%) of 72 samples of laryngeal squamous cell carcinoma.

Morshed - Pol Merkur Lekarski 2005 abstract / PubMed

Immunohistochemical demonstration of multiple HPV types in laryngeal squamous cell carcinoma. K Morshed, E Korobowicz, M Szymanski, D Skomra, W Golabek. Eur Arch Otorhinolaryngol 2005 Nov;262(11):917-920. 6/40 (15%) of laryngeal squamous cell carcinoma patients were positive for HPV, versus zero of 33 controls with with non-neoplastic laryngeal lesions or laryngeal nodules. "Five (83.4%) of six patients with HPV positive tumors had G2 (moderately differentiated), one patient (16.6%) had G3 (poorly differentiated), and no patient with HPV positive tumor had a G1 (well-differentiated) tumor. Four (66.6%) of the six HPV positive tumors were in the supraglottic region, one (16.6%) tumor was located in the glottis, and one (16.6%) HPV positive tumor was in the subglotic region."

Morshed - Eur Arch Otorhinolaryngol 2005 abstract / PubMed

Human papillomavirus and head and neck cancer: a systematic review and meta-analysis. CG Hobbs, JA Sterne, M Bailey, RS Heyderman, MA Birchall, SJ Thomas. Clin Otolaryngol 2006 Aug;31(4):259-266. "The association between HPV16 and cancer was strongest for tonsil (OR: 15.1, 95% CI: 6.8-33.7), intermediate for oropharynx (OR: 4.3, 95% CI: 2.1-8.9) and weakest for oral (OR: 2.0, 95% CI: 1.2-3.4) and larynx (OR: 2.0, 95% CI: 1.0-4.2). To investigate heterogeneity, further stratification by method of HPV16 detection, suggested that variation in the magnitude of the HPV-cancer association with cancer site was restricted to studies using ELISA: among studies using PCR, the magnitude of the summary odds ratios was similar across the four sites."

Hobbs - Clin Otolaryngol 2006 abstract / PubMed

Human papillomavirus and Epstein-Barr virus infection, p53 expression, and cellular proliferation in laryngeal carcinoma. DE de Oliveira, MM Bacchi, RS Macarenco, JV Tagliarini, RC Cordeiro, CE Bacchi. Am J Clin Pathol 2006 Aug;126(2):284-293. In 110 squamous cell carcinomas of the larynx, "High-grade HPV was found in 37.3% of cases, and none had demonstrable EBV infection."

de Oliveira - Am J Clin Pathol 2006 abstract / PubMed

Wart Virus Linked to Head and Neck Squamous Cell Carcinoma: Presented at AHNS. By John Otrompke. Doctor's Guide, Aug. 22, 2006. Presentation title: Frequency and Types of Human Papilloma Virus in Head and neck Squamous Cell Carcinoma. Poster 160,presented at the 2006 annual meeting of the American Head and Neck Society (AHNS), by Jose-Francisco Gallegos-Hernandez. In 118 head and neck cancer patients, HPV was found in 42% of the cases, 70% of which were HPV16. "Fifty percent of patients with laryngeal cancer had HPV, he said. HPV type 16 was present in 20% of those with mouth cancer, 25% of those with cancer of the mucosae, and 66% of those with cancer of the palate, while no other forms of HPV were found in patients with those forms of cancer in the study, the poster said. HPV was found more frequently in patients over 50 years of age and in men."

Wart Virus Linked to Head and Neck Squamous Cell Carcinoma, Aug. 22, 2006 / Doctor's Guide

Detection of human papillomavirus and relevant tumor suppressors and oncoproteins in laryngeal tumors. ME Manjarrez, R Ocadiz, L Valle, C Pacheco, A Marroquin, C De la Torre, M Selman, P Gariglio. Clin Cancer Res 2006 Dec 1;12(23):6946-6951. 16 laryngeal carcinomas versus 13 individuals who died of nonmalignant causes. "Most laryngeal papillomatosis samples contained low-risk HPV determined by both techniques. However, 25% of laryngeal carcinoma samples were positive for HPV employing PCR or in situ PCR." "In laryngeal cancer, we found that in situ PCR was more sensitive than in vitro PCR, detecting HPV-DNA in 25% of the samples as compared with 12.5% when in vitro PCR was employed. The large difference in the percentages reported by other authors can be explained partly by the different detection methods used." "Two cases with laryngeal cancer showed high-risk and low-risk HPV whereas the other two contained either low-risk HPV or high-risk HPV, suggesting that both low-risk and high-risk HPV might be involved in the development of laryngeal malignant tumors in a subset of patients, and that these viruses may be a synergistic risk factor for malignant development." Low-risk HPV was present in all papillomatosis patients.

Manjarrez / Clin Cancer Res 2006 full article (pdf, 6 pp)

[Human papillomavirus: association with head and neck cancer.] JF Gallegos-Hernández, E Paredes-Hernández, R Flores-Díaz, G Minauro-Muñoz, T Apresa-García, DM Hernández-Hernández. Cir Cir 2007 May-Jun;75(3):151-155. "Results: There were 118 patients were HPV positive and oropharyngeal and laryngeal cancer patients were the most frequently affected (55% and 50%, respectively). HPV-16 was most frequently isolated (70%). Laryngeal cancer patients suffered the highest ratio of HPV-16 infection (68.7%). Factors associated with HPV (univariate analysis) were age >50 years, tobacco/alcohol consumption and male gender. In multivariate analysis, none of the variables showed importance (p >0.5); HPV infection was more frequent in patients with history of alcohol/tobacco consumption (p = 0.6)."

Gallegos-Hernández -Cir Cir 2007 abstract / PubMed

Human papilloma virus prevalence in laryngeal squamous cell carcinoma. A Gungor, H Cincik, H Baloglu, E Cekin, S Dogru, E Dursun. J Laryngol Otol 2007 Aug;121(8):772-774. "Human papilloma virus deoxyribonucleic acid was detected in seven of 95 cases of laryngeal squamous cell carcinoma (7.36 per cent). Human papilloma virus genotyping revealed double human papilloma virus infection in three cases and single human papilloma virus infection in the remaining four cases. The human papilloma virus genotypes detected were 6, 11 and 16 (the latter detected in only one case)."

Gungor - J Laryngol Otol 2007 abstract / PubMed

Alcohol, smoking and human papillomavirus in laryngeal carcinoma: a Nordic prospective multicenter study. WJ Koskinen, K Brøndbo, H Mellin Dahlstrand, T Luostarinen, T Hakulinen, I Leivo, A Molijn, WG Quint, T Røysland, E Munck-Wikland, AA Mäkitie, I Pyykkö, J Dillner, A Vaheri, LM Aaltonen. J Cancer Res Clin Oncol 2007 Sep;133(9):673-678. Fresh-frozen laryngeal cancer biopsies from 108 patients in Finland, Norway, and Sweden. Four (3.3%) were positive for HPV, type(s) not specified in abstract.

Koskinen - J Cancer Res Clin Oncol 2007 abstract / PubMed

Lack of association of alcohol and tobacco with HPV16-associated head and neck cancer. KM Applebaum, CS Furniss, A Zeka, MR Posner, JF Smith, J Bryan, EA Eisen, ES Peters, MD McClean, KT Kelsey. J Natl Cancer Inst 2007 Dec 5;99(23):1801-1810. 485 cases (93 laryngeal tumors) and 549 controls. 80.7% of laryngeal cancer patients were negative for HPV16 by serology, which is neither reliable nor complete. Four of the authors were from the Harvard School of Public Health, and it was funded by the National Institutes of Health.

Applebaum / J Natl Cancer Inst 2007 full article

High incidence of malignant transformation of laryngeal papilloma in Taiwan. LA Lee, AJ Cheng, TJ Fang, CG Huang, CT Liao, JT Chang, HY Li. Laryngoscope 2008 Jan;118(1):50-55. 26 consecutive laryngeal papilloma patients. "During 237 person-years of follow-up, six new, pathologically confirmed cases of laryngeal carcinoma were ascertained (incidence 2.5/100 person-years), and all were associated with HPV-6 or HPV-11. Malignant transformation revealed no correlation with the following: age less than 3 years at diagnosis, sex, history of tobacco use, history of alcohol consumption, family history of laryngeal cancer, or type of laryngeal papilloma. Laryngeal papilloma without demonstrable HPV DNA was the only significant risk factor for malignant transformation (P < .05). The cumulative risk of malignant transformation in subjects without demonstrable HPV DNA was significantly higher than that in HPV-positive patients (relative risk, 8.0; 95% confidence interval, 1.1-60.3; P = .05)."

Lee - Laryngoscope 2008 abstract / PubMed

Short-fragment PCR assay for highly sensitive broad-spectrum detection of human papillomaviruses in laryngeal squamous cell carcinoma and normal mucosa: clinico-pathological evaluation. K Morshed, M Polz-Dacewicz, M Szymański, D Polz. Eur Arch Otorhinolaryngol 2008 Jul;265 Suppl 1:S89-96. 93 primary laryngeal squamous cell carcinomas, 49 specimens of normal mucosa, and 22 specimens of laryngeal nodules serving as controls. By SPF(10) HPV DNA test, PCR/DEIA method and INNO-LiPA genotyping assay, "Human papillomavirus was detected in 33 (35.5%) of the 93 samples from LSCC, in 4 (8.2%) of 49 samples of the normal mucosa and it was not detected in any of the sample from the control group. Twenty-eight of 33 (81.8%) were positive for HPV-16, 6 of 33 (18.2%) were positive for HPV-18 and 5 of 33 (15.1%) were positive for HPV-33. Multiple infection was found in 5 of 33 (15.1%); 3 samples were positive for HPV-16 and HPV-33, 2 samples for HPV-16 and HPV-18." "The difference between the presence of HPV in squamous cell carcinoma tumors and in control group samples was statistically significant (χ2 = 8.13; P = 0.004). The difference between the presence of HPV in squamous cell carcinoma tumors and in normal mucosa from the surgical margin in patients with LSCC samples was statistically significant (χ2 = 3.78; P = 0.05)." In Table 1, 32.5% of carcinomas in smokers were HPV-positive, versus 36.4% in non-smokers (P = 0.48).

Morshed - Eur Arch Otorhinolaryngol 2008 full article / PubMed Central

Retraction: WITHDRAWN: Human papillomavirus detection and genotyping in squamous cell carcinomas of the larynx. AM Silva, CA Vilanova-Costa, SF de Oliveira, CC da Silva, MP Curado, AD da Cruz. J Virol Methods 2009 May;157(2):231. In 20 cases, "HPV DNA was amplified in 25% of cases, and the amplified products from the first PCR were genotyped using specific primers for HPVs 6, 11, 16, 18, 33, 35, 45 and 58. HPV33 genome was found in three samples and HPV45 in two samples as the viral types associated most frequently with laryngeal cancer. HPVs 11, 16, and 18 were also genotyped. Three patients had two viral types simultaneously, suggesting that co-infection may play an important role in the etiology of cancer of the larynx."

Vilanova-Costa - J Virol Methods 2008 abstract / PubMed

Detection of HHV-8 and HPV in laryngeal carcinoma. MG Güvenç, K Midilli, A Ozdoğan, E Inci, R Tahamiler, O Enver, G Sirin, S Ergin, M Kuşkucu, EO Divanoğlu, G Yilmaz, K Altas. Auris Nasus Larynx 2008 Sep;35(3):357-362. 47 squamous cell carcinomas (SCC) and 3 verrucous carcinomas. "HPV DNA was detected in seven patients (7/50; 14%) (5 out of 47 patients with SCC (5/47; 10.6%) and two out of three patients with verrucous carcinoma). HHV-8 DNA was detected in five patients and they all had SCC (5/47; 10.6%). One case had both HHV-8 and HPV DNA. None of the control samples from cadavers harbored HHV-8 DNA, or HPV DNA. There was a statistically significant correlation between HHV-8 DNA and HPV DNA positivity and laryngeal SCC (Fisher exact test; p=0.023 for each). No statistically significant correlations were found between the presence of HHV-8 and/or HPV and age, gender, tumor stage, differentiation, the site of the tumor, smoking and alcohol use."

Güvenç - Auris Nasus Larynx 2008 abstract / PubMed

Use of in situ hybridization to detect human papillomavirus in head and neck squamous cell carcinoma patients without a history of alcohol or tobacco use. WT Lee, RR Tubbs, AM Teker, J Scharpf, M Strome, B Wood, RR Lorenz, J Hunt. Arch Pathol Lab Med 2008 Oct;132(10):1653-1656. 22 patients who did not use alcohol or tobacco, 14 male, 8 female; tongue (n = 8), tonsil (n = 7), and larynx (n = 7). "Only 2 cases were positive for high-risk HPV, and both demonstrated an integrated pattern. Both cases were tumors of the tonsil. No cases were positive for low-risk HPV."

Lee - Arch Pathol Lab Med 2008 abstract / PubMed

Galectin-3 overexpression in invasive laryngeal carcinoma, assessed by computer-assisted analysis. FA Miranda, MK Hassumi, MC Guimarães, RT Simões, TG Silva, RC Lira, AM Rocha, CT Mendes Jr, EA Donadi, CP Soares, EG Soares. J Histochem Cytochem 2009 Jul;57(7):665-673. "[P]araffin-embedded larynx biopsies from 65 patients, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, and 28 with metastases. Twenty-eight cervical lymph nodes from patients with metastatic lesions were also evaluated." 7 (12.7%) out of 55 patients were HPV positive.

Miranda - J Histochem Cytochem 2009 abstract / PubMed

Human papillomavirus in early laryngeal carcinoma. JL Baumann, S Cohen, AN Evjen, JH Law, S Vadivelu, A Attia, JS Schindler, CH Chung, PS Wirth, CJ Meijer, PJ Snijders, WG Yarbrough, RJ Slebos. Laryngoscope 2009 Aug;119(8):1531-1537. 38 cases of T1 or carcinoma in situ (CIS) laryngeal lesions by PCR for 37 HPV subtypes. "HPV DNA was detected in 6 (16%) of the 38 lesions, representing HPV types 16, 26, 31, 39, and 52,... This HPV prevalence is higher than that noted in many previous laryngeal cancer studies, possibly due to the relatively large panel of subtypes screened for in this study."

Baumann - Laryngoscope 2009 abstract / PubMed

Papillary squamous cell carcinoma of the head and neck: frequent association with human papillomavirus infection and invasive carcinoma. VY Jo, SE Mills, MH Stoler, EB Stelow. Am J Surg Pathol 2009 Nov;33(11):1720-1724. 33% of laryngeal tumors were HPV-positive by ISH.

Jo - Am J Surg Pathol 2009 abstract / PubMed

Prevalence of high-risk human papillomavirus types (HPV-16, HPV-18) and their physical status in primary laryngeal squamous cell carcinoma. B Liu, Z Lu, P Wang, Z Basang, X Rao. Neoplasma 2010;57(6):594-600. "Using HPV L1 general primer amplification, HPV DNA was detected in 23 (27.4%) of the 84 LSCC samples. When PCR products were cloned and sequenced, HPV16 were found in all 23 L1 positive samples. However, when specific primers for HPV- 16 or -18 were used to amplify E6 and E7 in all samples, 29 cases (34.5%) were positive for HPV-16, while 6 cases (7.1%) were positive for HPV 18. Coinfection of HPV-16 and -18 were found in 4 cases (4.8%). In addition, qRT-PCR assay found that HPV-16 was characterized as episomal in 51.7% of cases, mixed (i.e., episomal and integrated) in 34.5%, and integrated in 13.8%, while HPV-18 was similarly characterized as episomal in 83.3% of cases and mixed in 16.7%. In the present study, about 36.9% of patients with LSCC were found to be infected with HPV-16 and -18 and integration of HPV-16 and -18 DNA into the host genome was found."

Liu - Neoplasma 2010 abstract / PubMed

Usefulness and efficiency of formalin-fixed paraffin-embedded specimens from laryngeal squamous cell carcinoma in HPV detection by IHC and PCR/DEIA. K Morshed, M Polz-Dacewicz, M Szymański, A Smoleń. Folia Histochem Cytobiol 2010 Sep 30;48(3):398-402. "HPV was detected by two methods (SPF10 PCR/DEIA and IHC) in 14 (25.92%) out of 54 specimens from FFPE. Significant differences were found between the HPV detection using PCR/DEIA method and IHC method in FFPE tissues. The comparative analysis of the 54 samples after assuming PCR method in FFPE tissues showed accuracy of 92.6%, sensitivity of 90.5% and specificity of 93.9%."

Morshed - Folia Histochem Cytobiol 2010 abstract / PubMed

Detection of novel Human papilloma virus type 82 in laryngeal cancer: case report. G Pannone, F Sanguedolce, A Santoro, P Fierro, M Panetti, D Fierro, F Maiello, S De Maria, A Giannattasio, R Serpico, L Lo Muzio, S Metafora, P Bufo. Auris Nasus Larynx 2010 Oct;37(5):648-650. "We report the case of a 51-year-old man affected by laryngeal squamous cell carcinoma; analysis of DNA extracted by cancer cells by an innovative molecular virology assay (INNO-LiPA HPV Genotyping Extra) showed the presence of two high-risk HPV genotypes, HPV-73 and -82. Immunohistochemical examination confirmed positivity for both capsid protein and viral oncogenic protein E7."

Pannone - Larynx 2010 abstract / PubMed

Human papillomavirus infections in laryngeal cancer. MC Torrente, JP Rodrigo, M Haigentz Jr, FG Dikkers, A Rinaldo, RP Takes, J Olofsson, A Ferlito. Head Neck 2011 Apr;33(4):581-586. Review. "[A]pproximately 25% of laryngeal squamous cell carcinomas harbor HPV infections on meta-analysis, with common involvement of high-risk HPV types 16 (highest frequency) and 18. Preliminary results suggest that these high-risk HPV infections seem to be biologically relevant in laryngeal carcinogenesis, manifested as having viral DNA integration in the cancer cell genome and increased expression of the p16 protein."

Torrente - Head Neck 2011 abstract / PubMed

Low human papillomavirus prevalence in head and neck cancer: results from two large case-control studies in high-incidence regions. KB Ribeiro, JE Levi, M Pawlita, S Koifman, E Matos, J Eluf-Neto, V Wunsch-Filho, MP Curado, O Shangina, D Zaridze, N Szeszenia-Dabrowska, J Lissowska, A Daudt, A Menezes, V Bencko, D Mates, L Fernandez, E Fabianova, T Gheit, M Tommasino, P Boffetta, P Brennan, T Waterboer. Int J Epidemiol 2011 Apr;40(2):489-502. From two separate populations, in Central Europe and Latin America. "HPV16 E7 DNA prevalence among cases was 3.1% (6/196), including 4.4% in the oropharynx (3/68), 3.8% in the hypopharynx/larynx (3/78) and 0% among 50 cases of oral cavity carcinomas. Positivity for both HPV16 E6 and E7 antibodies was associated with a very high risk of oropharyngeal cancer (OR = 179, 95% CI 35.8-899) and hypopharyngeal/laryngeal cancer (OR = 14.9, 95% CI 2.92-76.1)."

Ribeiro - Int J Epidemiol 2011 abstract / PubMed

Human Papillomavirus Outcomes in an Access-to-Care Laryngeal Cancer Cohort. JK Stephen, KM Chen, V Shah, S Havard, M Lu, VP Schweitzer, G Gardner, MJ Worsham. Otolaryngol Head Neck Surg 2012 May;146(5):730-738. 79 patients with primary LSCC. HPV16 was detected in 27% of LSCC patients. There were no differences in survival between HPV-positive and HPV-negative patients.

Stephen - Otolaryngol Head Neck Surg 2012 abstract / PubMed

Possible implication of Mdm2 as a prognostic marker in invasive laryngeal carcinoma. MK Hassumi-Fukasawa, FA Miranda-Camargo, MC Guimarães, RT Simões, EA Donadi, CP Soares, EG Soares. Eur Arch Otorhinolaryngol 2012 Jul;269(7):1795-1804. 7 (13.2%) out of 53 patients were positive for HPV 6, 11, 16, 18, 31 or 33 by PCR.

Hassumi-Fukasawa - Eur Arch Otorhinolaryngol 2012 abstract / PubMed

Human Papillomavirus Infection and Laryngeal Cancer Risk: A systematic review and meta-analysis. X Li, H Li, J Gao, Y Yang, F Zhou, C Gao, M Li, Q Jin, L Gao. J Infect Dis 2013 Feb 1;207(3):479-488. 55 eligible studies. "The overall HPV prevalence in laryngeal cancer tissues was 28.0% (95% confidence interval [CI], 23.5%-32.9%). A total of 26.6% laryngeal cancer patients were infected with high-risk HPV types only, and HPV-16 was most frequently observed type, with a prevalence of 19.8% (95% CI, 15.7%-24.6%). The meta-analysis based on 12 eligible case-control studies suggests a strong association between HPV infection and laryngeal squamous cell carcinoma, with a summary odds ratio (OR) of 5.39 (95% CI, 3.25-8.94). Different magnitudes of association were observed for HPV-16 (OR, 6.07; 95% CI, 3.44-10.70) and HPV-18 (OR = 4.16; 95% CI, .87-20.04; P < .01)."

Li - J Infect Dis 2013 abstract / PubMed
Li / J Infect Dis 2013 full article

[Analysis of the human papillomavirus in laryngeal squamous cell carcinoma patients]. W Wei, YT Song, BY Zhang, JY Sun, C Chen, NS Zhang, XP Dong, Q Shi. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2013 Feb;27(1):22-24. 64 clinical diagnosed laryngeal squamous cell carcinoma patients. "In the 64 cases, 7 were positive for HPV infection by Luminex and PCR tests. 18 were positive for HPV16/18 E6 protein expression. The total positive rate was about 39. 1%."

Wei - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi 2013 abstract / PubMed

The role of human papillomavirus in head and neck cancer in Senegal. C Ndiaye, L Alemany, Y Diop, N Ndiaye, MJ Diémé, S Tous, JE Klaustermeier, M Alejo, X Castellsagué, FX Bosch, H Trottier, S Sanjosé. Infect Agent Cancer 2013 Apr 17;8(1):14. 3 of 25 (8.6%) laryngeal cancers were positive for HPV (Table 2).

Ndiaye - Infect Agent Cancer 2013 full article / PubMed Central

Biological evidence for a causal role of HPV16 in a small fraction of laryngeal squamous cell carcinoma. G Halec, D Holzinger, M Schmitt, C Flechtenmacher, G Dyckhoff, B Lloveras, D Höfler, FX Bosch, M Pawlita. Br J Cancer 2013 Jul 9;109(1):172-183. 102 patients. "Ninety-two patients were valid in DNA analysis, of which 32 (35%) had at least one HPV DNA+ sample. Among the 29 single infections, 22 (76%) were HPV16, 2 (7%) HPV56 and 1 each (4%) HPV45, HPV53, HPV70, HPV11 and HPV42. Three cases harboured HPV16 with HPV33 (twice) or HPV45. Only 32% of HPV DNA+ findings were reproducible. Among HPV16 DNA+ L-SCC, 2 out of 23 (9%) had high viral loads, 5 out of 25 (21%) expressed E6*I mRNA and 3 out of 21 (14%) showed high p16(INK4a) and low pRb expression (all three HPV16 RNA-positive)..."

Halec - Br J Cancer 2013 abstract / PubMed

Human papillomavirus infection a favorable prognostic factor in laryngeal squamous cell carcinoma is associated with the expression of proliferating cell nuclear antigen. H Jiang, PF Lin. Pak J Med Sci 2013 Sep;29(5):1173-1177. 71 patients. "31 (43.7%) cases showed infection of HPV and 38 (53.5%) showed overexpression of PCNA. No significant difference of HPV status in clinicopathological features was found."

Jiang - Pak J Med Sci 2013 abstract / PubMed

Comprehensive analysis of HPV expression in laryngeal squamous cell carcinoma. T Gheit, B Abedi-Ardekani, C Carreira, CG Missad, M Tommasino, MC Torrente. J Med Virol 2014 Apr;86(4):642-646. "Four cases out of 32 (13%) were HPV DNA+: HPV 11 (n = 1), HPV 31 (n = 3), HPV 59 (n = 1). One double infection: HPV 11 and HPV 31. p16(INK4a) was overexpressed in three cases (9%) and COX-2 in 17 cases (53%). Two of four HPV DNA+ samples had E6*I mRNA for HPV 31 and overexpressed p16(INK4a) and COX-2."

Gheit - J Med Virol 2014 abstract / PubMed

[Infection rate of human papillomavirus and its association with laryngeal cancer in Chinese: a meta-analysis]. C Zhang, Z Deng, Y Chen, M Xie. Zhonghua Yi Xue Za Zhi 2014 Apr;94(16):1245-1251. "The overall prevalence of HPV was 32% [95% confidence interval (CI): 22%-44%] and the prevalence of high-risk HPV-16/18 and low-risk HPV-6/11 was 30% (95%CI: 24%-37%) and 12% (95%CI: 9%-17%) respectively in Chinese LC patients... Meta-analysis showed the overall HPV infection rate in the LC group was significantly higher than in the control group (OR = 15.87 (95%CI: 9.25-27.24); P < 0.01). Moreover, as compared with the control group, the patients in the LC group had a higher infection rate of HPV-16/18 (OR = 8.07 (95%CI: 5.67-11.48); P < 0.01)."

Zhang - Zhonghua Yi Xue Za Zhi 2014 abstract / PubMed

Human papillomavirus genotypes in a subset of head and neck squamous cell carcinoma. G Kaba, B Dzudzor, RK Gyasi, RH Asmah, CA Brown, W Kudzi, EK Wiredu. West Afr J Med 2014 Apr-Jun;33(2):121-124. 8 of 33 (24.2%) tumors from the larynx were positive for HPV. "The commonest site for HPV-associated HNSSC in Ghana is the larynx, rather than the oropharynx as reported in other studies."

Kaba - West Afr J Med 2014 abstract / PubMed

Prevalence of human papillomavirus infection in greek patients with squamous cell carcinoma of the larynx. S Laskaris, I Sengas, P Maragoudakis, E Tsimplaki, E Argyri, L Manolopoulos, E Panotopoulou. Anticancer Res 2014 Oct;34(10):5749-5753. 54 biopsies. "HPV DNA was detected in 18.5% (10/54) of laryngeal SCC; infection from high risk (hr) HPV and low risk (lr) HPV types was found in 16.7% (9/54) and 1.8% (1/54) of the samples, respectively. HPV 16 was the commonest type detected in 7.5% (4/54). The presence of HPV DNA was significantly associated with the absence of tobacco use (p=0.001) and poorly differentiated tumors (p=0.003)."

Laskaris - Anticancer Res 2014 abstract / PubMed

Human papillomavirus detection in head and neck squamous cell carcinoma. D Vietía, J Liuzzi, M Avila, Z De Guglielmo, Y Prado, M Correnti. Ecancermedicalscience 2014 Oct 23;8:475. 29.1% of 23 [?] squamous cell carcinomas of the larynx were positive for HPV.

Vietía - Ecancermedicalscience 2014 full article / PubMed Central

Epstein-barr virus and human papillomavirus infections and genotype distribution in head and neck cancers. Z Deng, T Uehara, H Maeda, M Hasegawa, S Matayoshi, A Kiyuna, S Agena, X Pan, C Zhang, Y Yamashita, M Xie, M Suzuki. PLoS One 2014 Nov 18;9(11):e113702. 8/50 (16.0%) hypopharyngeal, and 4/28 (14.3%) laryngeal tumors were positive for HPV DNA. 32/50 (64.0%) hypopharyngeal and 14/28 (50.0%) laryngeal tumors were positive for EBV DNA.

Deng - PLoS One 2014 full article / PubMed Central
Deng / PLoS One 2014 full article

Human papillomavirus infection in 674 chinese patients with laryngeal squamous cell carcinoma. Y Xu, S Liu, H Yi, J Wang, P Dong, X Li, S Yin. PLoS One 2014 Dec 23;9(12):e115914. 674 LSCC patients in Shanghai. "The rate of HPV infection among patients with LSCC was 4.9% (33/674). HPV infection was more common among nonsmokers (P<0.05), nondrinkers (P<0.05), and patients with supraglottic LSCC (P<0.05). Of the 33 HPV-positive patients, 28 (84.8%) were infected with HPV-16, 2 with HPV-18, 1 with HPV-31, 1 with HPV-33 and 1 with HPV-45."

Xu / PLoS One 2014 full article

Human papillomavirus prevalence in invasive laryngeal cancer in the United States. BY Hernandez, MT Goodman, CF Lynch, W Cozen, ER Unger, M Steinau, T Thompson, MS Saber, SF Altekruse, C Lyu, M Saraiya; HPV Typing of Cancer Workgroup. PLoS One 2014 Dec 29;9(12):e115931. 148 invasive laryngeal cancers from SEER database. "HPV DNA was detected in 31 of 148 (21%) invasive laryngeal cancers. Thirteen different genotypes were detected. Overall, HPV 16 and HPV 33 were the most commonly detected types. HPV was detected in 33% (9/27) of women compared with 18% (22/121) of men (p = 0.08)." Also, "While HPV 16 and 18 constituted half of HPV-positive cases occurring in men, among women, only 1 was HPV 16 positive and none were positive for HPV 18."

Hernandez / PLoS One 2014 full article

Prevalence of human papillomavirus in laryngeal and hypopharyngeal squamous cell carcinomas in northern Spain. JP Rodrigo, MA Hermsen, MF Fresno, RH Brakenhoff, F García-Velasco, PJ Snijders, DA Heideman, JM García-Pedrero. Cancer Epidemiol 2015 Feb;39(1):37-41. 1/62 laryngeal and 1/62 (1.6%) hypolaryngeal squamous cell carcinomas were positive for HPV, both HPV 16.

Rodrigo - Cancer Epidemiol 2015 abstract / PubMed

EBV, HSV, CMV and HPV in Laryngeal and Oropharyngeal Carcinoma in Polish Patients. D Polz-Gruszka, A Stec, J Dworzański, M Polz-Dacewicz. Anticancer Res 2015 Mar;35(3):1657-1661. 80 patients. "HPV was detected in 32.5% (22.5% were HPV 16), more often in laryngeal (36%) than in oropharyngeal carcinoma (26.6%). EBV was identified in 57.5%, HHV-1 in 7.5%, and CMV in 10% of patients. Co-infection with one or more viruses was detected in 30% of cases and most frequently it was co-infection with EBV and HPV (15%)."

Polz-Gruszka - Anticancer Res 2015 abstract / PubMed

Frequency and prognostic significance of p16INK4A protein overexpression and transcriptionally active human papillomavirus infection in laryngeal squamous cell carcinoma. RJ Young, D Urban, C Angel, J Corry, B Lyons, N Vallance, S Kleid, TA Iseli, B Solomon, D Rischin. Br J Cancer 2015 Mar 17;112(6):1098-1104. 7 / 324 (2.2%) of LSCC patients were positive for high-risk HPV RNA by ISH. [Other types not mentioned in abstract].

Young - Br J Cancer 2015 abstract / PubMed

HPV Infection and Anemia Status Stratify the Survival of Early T2 Laryngeal Squamous Cell Carcinoma. H Wang, Z Zhang, R Sun, H Lin, L Gong, M Fang, WH Hu. J Voice 2015 May;29(3):356-362. 32/318 (10.1%) laryngeal squamous cell carcinomas had high-risk HPV infection.

Wang - J Voice 2014 abstract / PubMed

HPV Involvement in Head and Neck Cancers: Comprehensive Assessment of Biomarkers in 3680 Patients. X Castellsagué, L Alemany, M Quer, G Halec, B Quirós, S Tous, O Clavero, L Alòs, T Biegner, T Szafarowski, M Alejo, D Holzinger, E Cadena, E Claros, G Hall, J Laco, M Poljak, M Benevolo, E Kasamatsu, H Mehanna, C Ndiaye, N Guimerà, B Lloveras, X León, JC Ruiz-Cabezas, I Alvarado-Cabrero, CS Kang, JK Oh, M Garcia-Rojo, E Iljazovic, OF Ajayi, F Duarte, A Nessa, L Tinoco, MA Duran-Padilla, EC Pirog, H Viarheichyk, H Morales, V Costes, A Félix, MJ Germar, M Mena, A Ruacan, A Jain, R Mehrotra, MT Goodman, LE Lombardi, A Ferrera, S Malami, EI Albanesi, P Dabed, C Molina, R López-Revilla, V Mandys, ME González, J Velasco, IG Bravo, W Quint, M Pawlita, N Muñoz, Sd Sanjosé, F Xavier Bosch; ICO International HPV in Head and Neck Cancer Study Group. J Natl Cancer Inst 2016 Jan 28;108(6):djv403. 1374 pharyngeal, 1264 OC, and 1042 laryngeal cancers from 29 countries. "HPV-AF estimates based on positivity for HPV-DNA, and for either HPV E6*I mRNA or p16(INK4a), were 22.4%, 4.4%, and 3.5% for cancers of the oropharynx, OC, and larynx, respectively, and 18.5%, 3.0%, and 1.5% when requiring simultaneous positivity for all three markers."

Castellsagué - J Natl Cancer Inst 2016 abstract / PubMed

Detection of human papillomavirus in laryngeal squamous cell carcinoma: Systematic review and meta-analysis. RR Gama, AL Carvalho, AL Filho, AP Scorsato, RV López, J Rautava, S Syrjänen, K Syrjänen. Laryngoscope 2016 Apr;126(4):885-893. 179 studies, with 7,347 cases. "Altogether, 1,830 (25%) cases tested HPV-positive considering all methods, with effect size of 0.269 (95% CI: 0.242 to 0.297; random-effects model). In meta-analysis stratified by the 1) HPV detection technique and 2) geographic study origin, the between-study heterogeneity was significant only for geographic origin (P = .0001). In meta-regression, the HPV detection method (P = .876) or geographic origin (P = .234) were not significant study-level covariates. Some evidence for publication bias was found only for studies from North America and those using non-polymerase chain reaction methods, with a marginal effect on adjusted point estimates for both."

Gama - Laryngoscope 2016 abstract / PubMed

Prevalence of human papillomavirus types and variants and p16(INK4a) expression in head and neck squamous cells carcinomas in São Paulo, Brazil. JC Betiol, L Sichero, HO Costa, LL de Matos, MA Andreoli, S Ferreira, SF Faraj, ES de Mello, JS Sobrinho, LG Brandão, CR Cernea, MA Kulcsar, FR Pinto, AJ Gonçalves, MB Menezes, L Silva, LM Rossi, RA Nunes, L Termini, LL Villa. Infect Agent Cancer 2016 May 4;11:20. 9/66 (13.7%) laryngeal cancers were HPV-positive.

Betiol - Infect Agent Cancer 2016 full article PubMed Central
Betiol / Infect Agent Cancer 2016 full article

Classification of TP53 mutations and HPV predict survival in advanced larynx cancer. A Scheel, E Bellile, JB McHugh, HM Walline, ME Prince, S Urba, GT Wolf, A Eisbruch, F Worden, TE Carey, C Bradford. Laryngoscope 2016 Sep;126(9):E292-E299. 58 patients."High-risk HPV was found in 20 of 52 (38.5%) tumors."

Scheel - Laryngoscope 2016 abstract / PubMed

Low etiologic fraction for human papillomavirus in larynx squamous cell carcinoma. M Taberna, C Resteghini, B Swanson, RK Pickard, B Jiang, W Xiao, M Mena, P Kreinbrink, E Chio, ML Gillison. Oral Oncol 2016 Oct;61:55-61. "HPV DNA was detected by Inno-LiPa in 54 of 404 (13.4%, 95% CI 10.2-17.1) evaluable samples but was confirmed by HPV type-specific qPCR in only 14 (3.5%, 95% CI 1.9-5.7). Only 7 of 404 (1.7%, 95% CI 0.7-3.5) LSCC were positive for HPV E6/E7 mRNA expression, including HPV16 (n=4) and 1 each for 11, 26 and 33."

Taberna - Oral Oncol 2016 abstract / PubMed

Oropharyngeal and laryngeal but not oral cancers are strongly associated with high-risk human papillomavirus in 172 Greek patients. E Tsimplaki, E Argyri, A Sakellaridis, E Kyrodimos, D Xesfyngi, E Panotopoulou. J Med Virol 2017 Jan;89(1):170-176. "The adjusted ORs for positivity to high-risk HPV infection for oropharyngeal and laryngeal cancer were 20.3 (95% CI: 1.7-250.1) and 22.8 (95% CI: 2.5-206.2), respectively."

Tsimplaki - J Med Virol 2017 abstract / PubMed

HPV in Larynx Squamous Cell Carcinoma: New Serotypes and Survival Study within 10-Year Follow-up. A Sánchez Barrueco, F González Galán, D Lora Pablos, JM Villacampa Aubá, C Ballestín Carcavilla, C Cenjor Español, C Almodóvar Álvarez. Otolaryngol Head Neck Surg 2017 Apr;156(4):677-682. 123 samples from the glottis, between 1977 and 2005. "HPV DNA was detected in 22.76% (n = 28) of the samples. Eleven genotypes were detected, 2 of which had never been described in the larynx (HPV43 and HPV62). No increasing trend of HPV was observed over time."

Sánchez Barrueco / Otolaryngol Head Neck Surg 2017 full article

Frequency of Human Papillomavirus (HPV) 16 and 18 Detection in Paraffin- Embedded Laryngeal Carcinoma Tissue. SZ Hosseini, M Makvandi, A Samarbafzade, A Timori, N Ranjbar, N Saki, N Nisi, T Shahani, M Varnaseri, K Angali Ahmadi. Asian Pac J Cancer Prev 2017 Apr 1;18(4):889-893. "Overall 14/50 (28%) were positive for HPVs, 8 (18%) with HPV-16 and 6 (12%) with HPV-18. Additionally, 2 (4%) mixed infections of HPV 16 and 18 genotypes were observed among these cases."

Hosseini - Asian Pac J Cancer Prev 2017 abstract / PubMed

HPV16 DNA and integration in normal and malignant epithelium: Implications for the etiology of laryngeal squamous cell carcinoma. X Chen, L Gao, EM Sturgis, Z Liang, Y Zhu, X Xia, X Zhu, X Chen, G Li, Z Gao Z. Ann Oncol 2017 May 1;28(5):1105-1110. "The prevalence of HPV16 was higher in cases than controls (20 [6.7%] vs 8 [2.7%], aOR 2.84, 95% CI 1.21-6.68). The risk of LSCC associated with HPV16 DNA positivity was even higher in patients aged 55 years or younger (aOR 3.52, 95% CI 1.07-11.54), males (aOR 4.74, 95% CI 1.33-16.90), never-smokers (aOR 5.57, 95% CI 1.41-22.10), and never-drinkers (aOR 3.72, 95% CI 1.09-12.72). HPV DNA was partly or fully integrated in all 20 HPV16-positive cases but was episomal in all 8 HPV16-positive controls; however, the HPV16-positive cases and controls had similar viral loads ( P =0.28)."

Chen - Ann Oncol 2017 abstract / PubMed

Detection of High-Risk Human Papillomavirus Genotypes 16 and 18 in Head and Neck Squamous Cell Carcinomas in Jordan. L Abu Qatouseh, I Sabri, I Alkhatib, E Atwa, T Arafat. Asian Pac J Cancer Prev 2017 May 1;18(5):1337-1341. 6% of 43 laryngeal squamous cell carcinomas were positive for HPV-16/18.

Abu Qatouseh / Asian Pac J Cancer Prev 2017 full article (pdf)

The presence and prognostic significance of human papillomavirus in squamous cell carcinoma of the larynx. E Erkul, I Yilmaz, G Narli, MA Babayigit, A Gungor, D Demirel. Eur Arch Otorhinolaryngol 2017 Jul;274(7):2921-2926. "HPV DNA was detected in 19 of 73 (26.02%) in patients with laryngeal squamous cell carcinoma. Human papilloma virus genotyping revealed double human papilloma virus in one case (types 16 and 59) and HPV 16 in the remaining cases."

Erkul - Eur Arch Otorhinolaryngol 2017 abstract / PubMed

Prevalence of HPV infection in hypopharyngeal and laryngeal squamous cell carcinoma at Thailand's largest tertiary referral center. W Pongsapich, N Eakkasem, S Siritantikorn, P Pithuksurachai, K Bongsabhikul, C Chongkolwatana. Infect Agent Cancer 2017 Nov 2;12:58. 0/80 cases had oncogenic HPV types.

Pongsapich - Infect Agent Cancer 2017 full article / PubMed Central
Pongsapich / Infect Agent Cancer 2017 full article

Prevalence of human papillomavirus in benign and malignant laryngeal lesions in Egyptian patients: Cross-sectional study. P Milad, H Kassamy, A Askoura, S Abuelela, R Salem, D Ragab. Clin Otolaryngol 2018 Feb;43(1):312-316. 2 of 56 squamous cell carcinomas (3.6%) were HPV-positive.

Milad - Clin Otolaryngol 2018 abstract / PubMed

Comparative assessment of HPV, alcohol and tobacco etiological fractions in Algerian patients with laryngeal squamous cell carcinoma. carcinoma. N Kariche, MT Hortal, S Benyahia, L Alemany, N Moulaï, O Clavero, M Muñoz, W Ouahioune, D Djennaoui, C Touil-Boukoffa, S de Sanjosé, M Bourouba. Infect Agent Cancer 2018 Mar 13;13:8. 49 tumors. "We observed that 40.8% of all LSCC cases were associated with smoking, 40.8% had combined tobacco and alcohol exposure history, and 14.3% did not show prior exposure to either risk factor. 1 out of 3 in-situ carcinoma cases was positive for HPV-6. HPV prevalence was null in the invasive tumors. HPV DNA was detected in 2.38% for all studied cases. 10.2% of LSCC patients did not associate with any of the studied risk factors."

Kariche - Infect Agent Cancer 2018 full article / PubMed Central
Kariche / Infect Agent Cancer 2018 full article

The role of human papillomavirus in laryngeal cancer in Southern China. EWH Lam, MMH Chan, CKC Wai, CM Ngai, Z Chen, MCS Wong, ACM Yeung, JHM Tong, ABW Chan, KF To, PKS Chan. J Med Virol 2018 Jun;90(6):1150-1159. 85 patients. "HPV DNA was detected in one patient (1.2%, 95%CI: 0.2-6.4%) who had glottic cancer and harbored HPV-6."

Lam - J Med Virol 2018 abstract / PubMed

Prevalence and Prognostic Significance of HPV in Laryngeal Squamous Cell Carcinoma in Northeast China. F Tong, J Gen, B Yan, H Lou, X Chen, C Duan, J He, S Zhang, H Xie, H Li, D Yuan, F Zhang, H Meng, L Wei. Cell Physiol Biochem 2018 Aug 22;49(1):206-216. 132 (62.6%) out of 211 patients were positive for HPV DNA... Among the 132 HPV+ patients, 28 (21.2%) were HPV-16 single infection.

Tong - Cell Physiol Biochem 2018 / PubMed

[Significance of HPV and cell cycle related proteins in basaloid squamous cell carcinoma of the larynx]. LF Cui, H Chang, HY Zhao, CL Qu, Y Gao, HG Liu. Zhonghua Bing Li Xue Za Zhi 2018 Sep 8;47(9):682-686. 29 cases. "HPV DNA was detected in 27.6% (8/29) of the laryngeal BSCC, and all were HPV16."

Cui - Zhonghua Bing Li Xue Za Zhi 2018 abstract / PubMed

Human papillomavirus infection in patients with laryngeal carcinoma. O Onerci Celebi, E Sener, S Hosal, M Cengiz, I Gullu, G Guler Tezel. BMC Cancer 2018 Oct 20;18(1):1005. "HPV was not detected in any of the 82 laryngeal cancer patients' laryngeal specimens, nor in any of the 11 archived laryngeal specimens with normal laryngeal mucosa via cISH."

Onerci Celebi / BMC Cancer 2018 full article

Infection and coinfection by human papillomavirus, Epstein-Barr virus and Merkel cell polyomavirus in patients with squamous cell carcinoma of the larynx: a retrospective study. JM Vazquez-Guillen, GC Palacios-Saucedo, LG Rivera-Morales, MV Alonzo-Morado, SB Burciaga-Bernal, M Montufar-Martinez, R Ortiz-Lopez, V Gonzalez-Villasana, AC Martinez-Torres, JC Serna-Hernandez, SJ Hernandez-Martinez, EE Castelan-Maldonado, A Zavala-Pompa, MS Montalvo-Bañuelos, R Garcia-Cabello, EC Sanchez-Fresno, C Rodriguez-Padilla. PeerJ 2018 Oct 24;6:e5834. "HPV DNA was detected in 93 (47.7%) specimens. HPV-11 was the most frequent with 68 cases (73.1%), and HPV-52 was the most frequently HR-HPV found with 51 cases, which corresponds to 54.8% of all HPV-positive specimens. EBV DNA was detected in 54 (27.7%) tumor tissue specimens of which 25 (46.3%) were in coinfection with HPV. MCPV DNA was detected only in 11 (5.6%) cases of which 5 (45.4%) were in coinfection with an HR-HPV." No associations with patient smoking habits were found.

Vazquez-Guillen - PeerJ 2018 full article / PubMed Central
Vazquez-Guillen / PeerJ 2018 full article

Prevalence of Human Papillomavirus Type-16 in Head and Neck Cancer Among the Chinese Population: A Meta-Analysis. L Guo, F Yang, Y Yin, S Liu, P Li, X Zhang, D Chen, Y Liu, J Wang, K Wang, Y Zhu, Q Lv, X Wang, X Sun. Front Oncol 2018 Dec 12;8:619. HPV prevalence was 28.5% (18.2-38.7%) in 1107 laryngeal cancers.

Guo - Front Oncol 2018 full article / PubMed Central
Guo / Front Oncol 2018 full article

Human Papillomavirus Detection in Head and Neck Squamous Cell Carcinomas at a Tertiary Hospital in Sub-Saharan Africa. E Aboagye, F Agyemang-Yeboah, BM Duduyemi, C Obirikorang. Scientific World Journal 2019 Apr 2;2019:2561530. 7/26 (27%) laryngeal cancers were HPV+.

Aboagye - Scientific World Journal 2019 full article / PubMed Central
Aboagye / Scientific World Journal 2019 full article

Association study of cell cycle proteins and human papillomavirus in laryngeal cancer in Chinese Population. L Cui, C Qu, H Liu. Clin Otolaryngol 2019 May;44(3):323-329. 332 patients. "HPV DNA was detected in 45 of 332 (13.55%) patients with LSCC, with HPV-16 being the predominant genotype. The presence of HPV-16 DNA was significantly associated with basaloid squamous cell carcinoma and cystic lymph node metastasis (P < 0.05)."

Cui - Clin Otolaryngol 2019 abstract / PubMed

Human papillomavirus-associated squamous cell carcinoma of the larynx or hypopharynx: Clinical outcomes and implications for laryngeal preservation. RT Hughes, WJ Beuerlein, SS O'Neill, M Porosnicu, TW Lycan, JD Waltonen, BA Frizzell, KM Greven. Oral Oncol 2019 Sep 16;98:20-27. 12 of 94 (13%) were positive for HPV DNA.

Hughes - Oral Oncol 2019 abstract / PubMed

Human papillomavirus infection is not associated with laryngeal squamous cell carcinoma in Taiwan. CG Huang, LA Lee, TJ Fang, HY Li, CT Liao, CJ Kang, TC Yen, KC Tsao, TC Chen, SR Shih. J Microbiol Immunol Infect 2020 Feb;53(1):79-86. 41 laryngeal carcinoma patients, 27 with vocal polyp, 20 with vocal fold leukoplakia. "The prevalence of HPV L1 DNA in the LSCC group was equivalent to that in the VP and VFL groups (7.3% vs. 7.4% vs. 10.0%; P = 0.929; effect size = 0.20). High-risk HPV DNA detected using in situ hybridization was relatively rare in all groups (2.6% vs. 5.3% vs. 0.0%; P = 0.636; effect size = 0.81)."

Huang - J Microbiol Immunol Infect 2020 abstract / PubMed

Prevalence and Detection of Sexually Transmitted Cases of Laryngeal Carcinoma. Al-Qudah MA, AF Al-Shaikh, HK Haddad, MA Elhassan, OB Elhassan, MN Dababneh, AW Zaitoun, NS Al Ghamdi, BY Al-Najjar. Head Neck Pathol 2020 Mar 7 [Epub ahead of print]. 52 LSCC samples. "By polymerase chain reaction (PCR), high-risk strains of HPV were detected in 15.4% of tissue samples. HPV-16 was found in 75% of the positive samples for an overall prevalence of 13.5%."

Al-Qudah - Head Neck Pathol 2020 abstract / PubMed

Prevalence of high-risk human papillomavirus and its genotype distribution in head and neck squamous cell carcinomas. Y Kim, YH Joo, MS Kim, YS Lee. J Pathol Transl Med 2020 Jul 21 [Epub ahead of print]. 5/43 (11.6%) laryngeal SCCs were positive for high risk HPVs.

Kim - J Pathol Transl Med 2020 abstract / PubMed
Kim / J Pathol Transl Med 2020 full article


Correlation of histology, human papillomavirus, and viral load in laryngeal papillomas of childhood. G Sharma, J DeHart, GJ Nuovo. Diagn Mol Pathol 2005 Dec;14(4):230-236. 29 of 47 (62%) of cases were positive for HPV, types 6 and 11, by ISH. There were less than 20 HPV-positive cells per tissue. 38 of 47 (81%) were positive by RT in situ PCR using primers specific for HPVs 6 and 11, in lesions negative by ISH.

Sharma - Diagn Mol Pathol 2005 abstract / PubMed

P16INK4A immunostaining is a strong indicator for high-risk-HPV-associated oropharyngeal carcinomas and dysplasias, but is unreliable to predict low-risk-HPV-infection in head and neck papillomas and laryngeal dysplasias. JJ Mooren, SE Gültekin, JM Straetmans, A Haesevoets, CJ Peutz-Kootstra, CU Huebbers, HP Dienes, U Wieland, FC Ramaekers, B Kremer, EJ Speel, JP Klussmann. Int J Cancer 2014 May 1;134(9):2108-2117. Paraffin-embedded tissue sections of 162 oropharyngeal squamous cell carcinomas (OPSCC), 14 tonsillar and 23 laryngeal dysplasias, and 20 tonsillar and 27 laryngeal papillomas. "P16INK4A immunohistochemistry revealed a strong nuclear and cytoplasmic staining in 50 out of 51 HPV16-positive and 5 out of 111 HPV-negative OPSCC (p<0.0001) and in all HPV16-positive tonsillar dysplasias, whereas highly variable staining patterns were detected in the papillomas and laryngeal dysplasias, irrespective of the HPV-status."

Mooren - Int J Cancer 2014 abstract / PubMed

Reliable detection of Human papillomavirus in recurrent laryngeal papillomatosis and associated carcinoma of archival tissue. D Weiss, T Heinkele, C Rudack. J Med Virol 2015 May;87(5):860-870. "[A]rchival tissue samples from 44 patients with RLP at laryngeal site, out of which eight developed laryngeal cancer, was screened for presence of HPV through various molecular approaches... The overall detection rates of HPV with the various methods used in this study were: HPV16 E6/E7 PCR: 0%; GP5+/6+ PCR: 4.5%; CDKN2A/p16 immunohistochemistry: 6.8%; in-situ hybridization for low and high risk HPV types: 52.3%; HPV6/11 L1 PCR: 72.7% and HPV6/11 E6 PCR: 79.5%. Disease progression showed no apparent dependence of the detected HPV type or clinical variables like age at diagnosis, sex, or additional drug application (Cidofovir and Bevacizumab). In conclusion, the broad-spectrum PCRs alone or in combination with immunohistochemistry of CDKN2A/p16 and in-situ hybridization are unsuitable for HPV detection in RLP. Based on the findings presented in this study the type specific PCRs targeting the E6 open reading frame are clearly superior in detection of HPV in this tumor entity."

Weiss - J Med Virol 2015 abstract / PubMed

Differential Diagnosis

Laryngeal zoster mimicking a laryngeal cancer. E Higuchi, Y Nakamaru, R Ohwatari, T Sakashita, Y Mesuda, A Homma, Y Furuta, S Fukuda. Otolaryngol Head and Neck Surgery 2005 Oct;133(4):1-3. The patient had a white ulcerated lesion on the left arytenoid and the epiglottis, and paralysis of the left vocal cord. Pathological examination showed "moderate to severe dysplasia highly suggestive of squamous cell carcinoma. However, severe inflammation precluded definitive pathological diagnosis, and rebiopsy was recommended. At this point, the most likely diagnosis was thought to be cancer of the larynx."

Higuchi / Otolaryngology–Head and Neck Surgery 2005 full article (pdf, 3 pp)

See Also:

Confounding By Infection - why studies that don't include full detection of HPV (and other causal infections) are defective, and falsely blame smoking and other non-causal associations.

The Lie That p53 Mutations Are the Mechanism Behind Lung Cancer - this is because p53 mutations happen after maligancy has occurred, and the point is relevant to other cancers as well.

HPV Infects Kids - HPV infection including oncogenic types is common in children
HPV Causes Head and Neck Cancers
HPV Causes Lung Cancer
HPV Causes Oral Cancer
HPV Strains and Oncogenicity
HPV Is Implicated in Esophageal Cancer

<= Back to The Tobacco Lawsuits

cast 08-06-20